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Microbiome

A more accurate assessment and monitoring of the efficacy of the microbiome can be achieved with an organoid model closely resembling the human intestine.

Microbiome

Lambda offers highly human-mimicking intestinal organoids, enabling realistic research not only for long-term studies of the gastrointestinal system but also for more lifelike investigations into the interactions between cells and microorganisms.
This platform is versatile, serving as a valuable tool for drug development and disease research.

Cell Type

· ASC

 

Organoid type

· Intestinal organoid

Lambda provides a 2D platform for gut attachment and allows for the evaluation of probiotics efficacy through gut attachment analysis.

Cell Type

· iPSC

 

Organoid type

· Intestinal organoid

Assay

Probiotic adhesion assay (2D)

Probiotics, known to exist in the gut, must possess the ability to survive in the body and exhibit high attachment capabilities to colon epithelial cells to exert various physiological functions in the human system.
Lambda provides a 2D platform for gut attachment and allows for the evaluation of probiotics efficacy through gut attachment analysis.

Production of beneficial substances
Metabolites with efficacy are produced.

Acid resistance, Bile resistance
Must survive to reach the intestine.

Intestinal colonization
Must attach to intestinal epithelial cells and colonize.

Discover the intricacies of intestinal colonization: Dive deeper into understanding the crucial role of microbial inhabitants in your gut health.
Key to successful probiotic formulation development: Gut colonization.

Generation of intestinal organoids
Existing Intestinal epithelium model

Caco-2 cell

  • Composed of single cells derived from colon cancer, not normal human cells
  • Composed of enterocytes with absorption function
  • Absence of Mucin Layer
The world’s first hPSC-derived intestinal epithelial model

Functional hIEC

  • Highly functional model based on high human body simulation
  • Provides precise efficacy and PK evaluation of microorganisms, drugs, etc.
  • Forms a sophisticated mucin layer composed of various absorption and secretion cells

Introducing our state-of-the-art Human Intestinal Epithelial Cell (hIEC) model, a significant advancement over traditional colorectal cancer cell models like Caco-2.
This innovative model replicates the diverse cell types found in the human intestine,
including enterocytes and goblet cells, creating a Mucus Layer remarkably similar to that in the human body.
This model exhibits a significant increase in mucin-related gene expression, critical for intestinal mucosa, underscoring
its immense potential as a key tool in gut health research and pharmaceutical development.

Assay process

  • Step 1: hPSC-derived human intestinal epithelial cell culture(differentiation)
  • Step 2: By culturing lactic acid bacteria and co-culturing them with intestinal epithelial cells, the bacteria adhere to the cells.
  • Step 3_1: Perform a CFU (Colony-Forming Unit) Assay to eliminate non-attached bacteria through washing, followed by the separation of cells, and assess viable bacterial activity.
  • Step 3_2: Fluorescent image analysis after removal of non-attached bacteria by washing

Technical

Dextran-FITC permeability assay

hIEC model is anticipated to play a crucial role in various fields, including drug development, gut health research, and disease modeling.
Utilizing this model, we have assessed drug absorption and permeability, and analyzed metabolites, yielding results more closely aligned with clinical data than those obtained with the traditional Caco-2 cell model.