Next-Generation Sequencing (NGS)

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Next-Generation Sequencing
(NGS)

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Next-Generation Sequencing (NGS)

Next-Generation Sequencing (NGS) is a rapid and efficient DNA sequencing technology used for analyzing multiple samples in bulk.
Widely applied in genomics, transcriptomics, and personalized medicine, NGS offers fast and accurate results.
At LAMBDA’s NGS center, our team of specialists utilizes the latest analytical technologies and extensive experience to deliver results that closely align with
researchers’ objectives.
We are dedicated to providing rapid and accurate results tailored to our customers’ research needs.

Eye Irritation test – HET-CAM assay

The degree of eye irritation to the cornea, iris, and conjunctiva isdetermined by observing bleeding, melting, and coagulation when the test substance is applied, inducing irritation to the blood vessels distributed in the chorioallantoic membrane of the fertilized.

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Process

Sample preparation

Requirements

Sample Type

DNA

Tumor Contents

–

Sample Condition

gDNA, ≥ 400ng
FFPE gDNA, ≥ 500ng

Cell Pellets

≥ 90%

1-2 x 106 cells

FFPE Tissue Block

≥ 30%

FFPE Tissue block (60μm thick, 250mm2)
+ 1 H&E slide

FFPE Tissue Slide

≥ 30%

10 slides (10μm thick) + 1 H&E slide)
6 slides (10μm thick, 250mm2 surface area)
+ 1 H&E slide
2-3 curls (30μm thick, 250mm2 surface area)

Frozen Tissue

≥ 30%

Tissue size (5mm x 5mm x 5mm)

*Titer is above 1012/ml

Shipping condition

Sample Type

DNA
FFPE
Frozen Tissue

Temperature

-80°C
Room Temperature
-80°C

Cooling Agents

Dry Ice
–
Dry Ice

Packing

Styrofoam Box

QC criteria

Sample Type

DNA

Amount (Qubit)

≥ 400ng

OD(Optical Density)

1.7 < 260/280 < 2.1
260/230 ≥ 1.6

*Concentration measured with nanodrop is unreliable. We recommend quantification using fluorometry.
*If quantification was performed using nanodrop, please prepare twice the minimum concentration.

Readout

Standard analysis report

Sequencing Information

Application
Material
Library
Type of Read
Read Length
Platform
Date of NGS Report
Analysis Institute
Result Folders and Files

Whole Exome Sequencing (WES)
DNA (gDNA)
Twist Exome 2.0
Paired-end
151
Illumina Novaseq
2023.11.29
Lambda Biologics
bam, fastq, maf, report, vcf

Variant annotation

Advanced analysis report

NGS Quality
Control Report

Somatic Variants
Profiles

OncoPlots

HLA Typing

Variants Profile
Concordance

Mutation Rate

Individual Matching

Microsatellite
Instability

Copy Number
Alteration