Adaptive Spatial Profiling Solution
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OPAL has the advantage of being able to stain freely without restrictions on the host of the primary antibody, making it easy to use antibodies already available in the laboratory and increasing experimental flexibility.
It is possible to stain up to 8 biomarkers at a time, and has the advantage of being relatively cheaper than CODEX staining.
Staining
2 days
Image
1-3 days per slide
Analyze
4 months
Immune Panel – 31 Markers
STEP core panel
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
CD4
CD68
CD20
CD11c
CD8
HLA-DR
CD3e
CD44
CD45
HLA-A
CD14
Ki67
Pan-CK
CD56
CD45RO
Helper T cells
Macrophages
B cells
Dendritic cells
Cytotoxic T cells
APCs (MHC II)
T cells
Activated T cells
Immune cells
MHC I
Monocytes
Proliferating cells
Tumor cells
NK cells
Memory T cells
Advanced Immune module
1
2
3
4
CD163
FoxP3
Granzyme B
CD21
M2 Macrophages
Regulatory T cells
Activated T cells / NK cells
Dendritic cells, mature B cells, FDCs
Immune activation module
1
2
3
4
5
PD-1
LAG-3
ICOS
PD-L1
IDO1
Checkpoint receptor
Checkpoint receptor
Checkpoint receptor
Checkpoint ligand
Multifunctional/Immune inhibitory
Structural module
1
2
3
4
5
6
7
E-cadherin
SMA
Vimentin
Collagen IV
CD31
CD34
Podoplanin
Epithelial cells
Vascular, Fibroblasts, Smooth muscle
Cyto-structures
Extracellular matrix
Vascular structures
Vascular structures
Lymphatics
Organ Categories
Brain
Brain
MAP2
LMX1A
FOXA2 or HNF-3β
N-Cadherin
NESTIN
NURR1
OTX2
FOXG1
PAX6
PLZF
Synapsin-1
Tyrosine hydroxylase
NeuN
NG2
GFAP
NF-M
Lyz
DPP10
Nestin
Neuronal nuclei
Neuronal Class III β-Tubulin
PAX6
Peripherin
Alpha Synuclein
SST
SOX2
Kidney
Kidney
WT1
PODXL
LTL
NPHS1
Liver
Cholangiocarcinoma
Hep Par1
CEA (1C7)
AFP
CA19-9
Liver
COL-1
KRT7
PDGFR
BSEP
ALB
Salivary
Salivary
CD24
Cytokeratin 5
Cytokeratin 7
Cytokeratin 14
Cytokeratin 19
Na+/K+-ATPase α1
NKCC1
CD44
Amylase
Aquaporin 5
c-Kit
S100A8
ITGA6
MIST-1
NGFR
CD166
Pancreas
Pancreas
annexin-13 (ANXA13)
ANXA1
ANXA10
TP53
Pancreatic Cancer
EGFR
CD73
Breast Cancer
Breast Cancer
HER2
TTF1
Cardiac
Cardiac
a-actin
cTnT
GJA1
CD31
aSMA
Cornea
Cornea
CDH2 (N-cadherin)
E-cadherin
Vimentin
ATP1A1
ZO-1
Gastritis
Gastritis
MUC6
LGR5
Lung
Lung Cancer
TTF-1/NKX2-1
XBP1s
p40 – DeltaNp63
BIG2
phospho-IRE1 (Ser724)
Cytokeratin 5
Cytokeratin 6
Cytokeratin 7
Skin
Skin
Loricrin
Vimentin
Cytokeratin 5
Cytokeratin 10
Cytokeratin 15
Cytokeratin 17
MMP1
Melanoma gp100
MiTF
Tyrosinase
PDGFRα
SOX2
P-cadherin
Ki-67
Human Nephronectin
E-Cadherin
TFAP2A
p75NTR
Collagen IV
LHX2
PMEL
TUBB3
LEF1
GFRA1
SOX10
ZO-1
Occludin
Skin_Hair
LHX2
SOX2
PDGFRa
CK17
CK5
PCAD
Intestine
Colon
CK20
β-catenin
CDX2
SMOC-2 (F-11)
Ets-2 (E-5)
DNA-PKCS(G-12)
EphB3(7E5)
Cytokeratin 20
Lysozyme
Lefty (F-11)
Mucin2 (F-2)
EphB2
CD24
Chromogranin A
Chr-A
Villin
Aquaporin 8
Aldehyde dehydrogenase 5
BTNL3
Cdk6
CDCA7
CEACAM7
Ephrin B1
HSP60
IEX1/IER3
Ki67
GPR49/LGR5
LGR5
NUPR1
MUCDHL
PTK7
RNF43
SLC26A3
TOMM40
ZG16
OLFM4
FCGBP
Other
Muc2
Lysozyme
Chromogranin A
LGR5
OLFM4
FCGBP
EPHB3
PLAC8
MUC2
KRT20
TOMM40
SLC26A3
ChgA
SOX9
SI
CD31
Vimentin
a-SMA
Emilin1
Pdx1
SIS(sucrase-isomaltase)
SLC40A1(Ferroportin)
Villin
ZO-1
Occludin
Ovary
Ovarian Cancer
CA125 (MUC16)
Ovary
ANXA10
HE(HE4)
WT1
HE4
CA125(MUC16)
PAX8
WT1
Retina
Retina
BRN3
Calbindin
Recoverin
Retinal RX
PAX6
CHX10
Liver organoid
H&E
OPAL
Merge
DAPI
ALB
ALB
PDGFR
BSEP
COL1
CD31
Brain organoid
H&E
OPAL
Merge
DAPI
NeuN
NF-M
MAP2
GFAP
SOX2
NG2
Colon organoid
H&E
OPAL
Merge
DAPI
ChaA
ECAD
SOX9
Lyz
Muc2
SI
Confirmation of similarity using OPAL staining in organoids derived from various organs undergoing differentiation.
OPAL
Merge
DAPI
OLFM4
SLC26A3
MUC2
FCGBP
KRT20
TMM40
Outcomes of organoid and co-culture cell analysis through OPAL staining
Lambda Biologics GmbH
Deutscher Pl. 5c, 04103 Leipzig, Germany
